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Each biopsy sample was analyzed at least twice in duplicate.
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The genomic DNA from 15 adenoma and 18 carcinoma prostate biopsy samples was analyzed by NotI-microarrays.
The same 117 homogenized biopsy samples were analyzed by wavelength-dispersive X-ray fluorescence to quantify the carbonate substitution in biological apatite as a function of age.
Each sample was analyzed in triplicate.
Each sample was analyzed twice.
Three representative cases are shown in Figure 2A, B, C. Because the sampling of tumor may lead to tissue specimens with different degree of "contamination" with non-malignant that may affect the result of methylation in the post-treatment, biopsies, all samples were analyzed by a pathologist.
After biopsy and volatile collection, melanoma samples were analyzed and confirmed by H&E staining.
Percutaneous renal biopsy was perfomed and tissue samples were analyzed by light microscopy (LM) and immunofluorescence (IF).
From each animal two samples were analyzed, biopsies near the carina and from peripheral lung tissue.
We made chromoendoscopy with magnification in the last 10 cm of the rectum and we took biopsies on the FAC sites; the samples were analyzed for a blind-test pathologist and we realized immunohistochemistry for p53 and Ki-67.
Samples were analyzed in triplicate.
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