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Biopsies were taken using a standardized protocol.
Female biopsies were taken using a crossbow and bolts fitted with biopsy tips (Quality Manufacturing , Inc.
On each CID, the anthropometrical and physiological parameters were measured, and blood, urine and fat biopsies were taken using the same standardized protocol at each centre [4].
Biopsies were taken using a standardized biopsy protocol, as previously described [ 17].
Biopsies were taken using a 14 G core needle under ultrasound guidance.
16 Mucosal biopsies were taken using a Radial Jaw three biopsy forceps (Boston Scientific, Natick, Massachusetts, USA).
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A total of four mucosal biopsy specimens were taken using standard biopsy forceps from all subjects from the rectosigmoid junction in order to standardise the site of sampling.
A total of 38 biopsy specimens were taken using NBI; 20 (53%) of them were identified histologically as endometriosis.
On average, three ultrasound-guided pre-treatment core biopsies of the primary tumour were taken using a 14 G core needle, after which two biopsies were snap-frozen in liquid nitrogen and stored at −80°C for molecular analysis and one biopsy was formalin-fixed and embedded in paraffin for classification and immunohistochemistry (IHC).
At the end of the sensitization protocol, mice were sacrificed and skin samples of the treatment area were taken using biopsy punches (kai medical; kai Europe GmbH, Solingen, Germany) from each mouse.
These sections were taken using needle biopsy, stained with hematoxylin and then magnified with an optical microscope.
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