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The liver biopsies were scored using the Nonalcoholic Steatohepatitis Clinical Research Network histologic scoring system.
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These 11 biopsies, plus 30 others (randomly selected from JDM patients without anti-MDA5 autoantibodies) were scored using the previously published and validated JDM biopsy score tool, (Table 2) [ 17, 18].
Alleles were scored using GeneMarker.
For comparison with the OsScore, sections were scored using a modified O'Driscoll score (MOD; ), selecting the properties that it was possible to measure on isolated biopsy specimens.
All patients underwent a systematic liver biopsy evaluation that was scored using the nonalcoholic steatohepatitis (NASH) Clinical Research Network (NASH-CRN) histological scoring system.
Liver biopsy performed within 6 months of the start of the study were used as baseline liver biopsy; otherwise, a percutaneous liver biopsy was performed and was scored using the Nonalcoholic Steatohepatitis-Clinical Research Network (NASH-CRN) hiscoringc systemg system.
Formalin-fixed, paraffin-embedded tumour biopsies were stained with anti-factor VIII using immunohistochemistry. Vascularity was scored using the intra-tumour microvessel density (IMD), or 'hot-spot', technique.
Thus using the C57BL/6N- Tyr cWTSI strain germline transmission can be scored using coat color with only black chimera progeny requiring biopsying and genotyping.
Liver biopsies were scored as 0 using the severity assessment scoring system for inflammatory lesions.
Biopsies were scored for cellularity and profiled using Agilent 180k human whole genome CGH arrays.
Biopsies were scored for inflammation and fibrosis, used for gene expression profiles, and tested for association with indicators of disease severity, response to surgery, and survival at 2 years.
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