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Biopsies were performed using a flexible biopsy forceps with a diameter of 3 Fr.
Biopsies were performed using standard biopsy forceps without needles (FB210K, Olympus co., Japan).
Core biopsies were performed using a 14-g automated gun using at least two core passes.
The liver biopsies were performed using a Trucut needle and were guided by ultrasound.
Biopsies were performed using the Suros ATEC biopsy system as previously described [ 31], and the incision site closed by suturing.
Biopsies were performed using an 18 G core biopsy needle, and an overall accuracy of 89% was achieved [ 15, 16].
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Genotyping of the mice from tail biopsies was performed using a PCR-based method, as previously described [9].
DNA extraction of mucosal biopsies was performed using the commercially available Qiagen QIAamp Mini kit (Qiagen Crawley UK) with the following amendments.
Amplification of genomic DNA extracted from tail biopsies was performed using Taq DNA polymerase (Fermentas AB, Vilnius, Lithuania) and a program of 1 min at 95°C, followed by 30 cycles at 95°C (30 s), 60°C (30 s), and 72°C (1 min).
Immunostaining of 5 µm sections from paraffin-embeded liver biopsies was performed using a combination of the following markers, Galectin-9 (goat-anti-human Galectin-9, 1∶100, R&D Systems), CD-68 or Albumin (mouse-IgG-anti CD68 or mouse-IgG-anti Albumin, 1∶100, Abcam) in combination with DAPI for nuclear staining (1∶20,000, Invitrogen).
RNA profiling in muscle biopsies was performed using a Human GE 4×44K v2 Microarray kit (Agilent Technologies, Massy, France).
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