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Before RNA isolation, biopsies were lysed using the MagNALyser (Roche Applied Science, Penzberg, Germany).
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Erythrocytes were lysed using a lysing solution (lysing buffer; BD Pharm Lyse™ BD Biosciencess, Japan) to segregate leukocytes.
Cells were lysed using RIPA buffer supplemented with protease inhibitors.
Then, red blood cells were lysed using ACK lysis buffer (Sigma, St . Louis MO, USA).
Cells were lysed using NP40 lysis buffer supplemented with PMSF and protease inhibitor cocktail.
Cells were lysed using Trizol (Invitrogen) and total RNA was collected.
Cells were lysed using a Dounce homogenizer.
For spleens, red blood cells were lysed using red blood cell lysing buffer (Sigma).
Cultured cells were lysed using radioimmunoprecipitation assay (RIPA) buffer.
Red blood cells were lysed using PharmLyse (BD Biosciences).
Upon thawing, cells were lysed using a sonication probe.
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