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Furthermore, the biopsies were graded using a simplified method for histological assessment of inflammation in UC [ 28], which includes crypt distortion (score 0 4), crypt inflammation (score 0 3) and subcryptal leukocyte infiltration (score 0 2) (maximum score was 7).
The tumor biopsies were graded according to Bergkvist (Table 1).
Salivary gland biopsies were graded according to Chisholm and Mason.
Small bowel biopsy histology was graded by using the system of Marsh.
Duodenal biopsies have been graded according to modified Marsh classification [ 19, 20], and immnuohistochemistry has been used to identify and count CD3+ intraepithelial lymphocytes.
Biopsies were homogenised using GENTLEMACS (Miltenyi Biotec) according to the manufacturer's recommendations.
Biopsies were taken using a standardized protocol.
Pretreatment grade of liver inflammation was graded on liver biopsy by using modified HAI [ 4].
Histological severity was graded from a biopsy adjacent to the study biopsy.
According to Brunt's criteria, liver biopsy with at least fat and lobular inflammation is graded as mild (grade 1), moderate (grade 2) or marked (grade 3) NASH.
