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The residual biopsies were fixed in Carnoy's fixative for up to 5 hours and then dehydrated, cleared and paraffin-embedded for histologic examination.
All colon biopsies were fixed in Bouin's fixative and processed routinely.
For histological examination, colon biopsies were fixed using standard processing on formalin-fixed paraffin embedded tissue.
The biopsies were fixed overnight in 4% neutral buffered formalin.
The biopsies were fixed overnight in 4% phosphate-buffered formaldehyde and transferred to alcohol 70%[38]].
Biopsies were fixed in formalin, embedded in paraffin, and cut into 4-μm-thick sections.
Brain biopsies were fixed in 4% paraformaldehyde and embedded in paraffin.
For expression in whole skin, the biopsies were fixed in 4% paraformaldehyde for 3 hours.
The biopsies were fixed in paraformaldehyde, embedded in paraffin and stored at room temperature until immunohistochemical analysis.
Autopsy material and skin biopsies were fixed in 4% phosphate-buffered formalin and embedded in paraffin as described before [33].
The biopsies were fixed in neutral buffered formalin and embedded in paraffin.
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