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All biopsies were completed using a standard ultrasound and biopsy probe with patients receiving prophylactic antimicrobials and intravenous sedation prior to their procedure.
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All computations were completed using STATA v10.0.
Core biopsies were obtained prior to initiation of chemotherapy, and cDNA analysis of RNA extracted from biopsy samples was completed using the HgU95-Av2 GeneChip (Affymetrix, Santa Clara, CA, USA).
Fine-needle muscle biopsies were completed at baseline, 1 hour, and 5 hours after exercise.
The subject then stayed in the clinic throughout the day until all three biopsies were completed.
Open biopsy and fine needle re-biopsy of the right inguinal lymph nodes were completed by the surgical service.
Questionnaires were completed by the oncologist before and after biopsy to determine whether the biopsy results changed the treatment plan.
The integrative sequencing and analysis were completed within 4 weeks after biopsy.
Paired biopsies have been completed in 42 patients, with paired genomic data before and after therapy obtained in 26 cases.
Bumetanide was added after EP agonist experiments on the biopsies had been completed.
Biopsies could not be completed for 22 (7.7%) blastocysts due to either poor morphology or degeneration after biopsy.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com