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Various biophysical methods have been used to characterize the photointermediate states in detail and to relate them to transport function (for reviews, see refs (3 and 12− 12−).
Biophysical methods have been utilized to assess the effects of missense mutations on the folding of the BRCA1 BRCT domain; however, they often cannot be used because of an inability to produce and purify many of the different variants.
While different biophysical methods have been used for fragment-based drug discovery including most often SPR or isothermal titration calorimetry (ITC) and various fragment optimization approaches, including SAR and structure-based design aided by computational or X-ray studies the pioneering work known as SAR by NMR described in section 4.1 is arguably the father of the current field.
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Several methods have been used to study thermal aspects of headgear, which could be categorized as (i) numerical, (ii) biophysical, (iii) combined numerical and biophysical, and (iv) user trials.
Further characterization of Sec10p by biophysical and structural methods has been hindered by the lack of soluble recombinant Sec10p protein.
This method has been extensively used to investigate the biophysical regulation of exocytosis and the dynamics of the fusion pore formed between the vesicle and the membrane.
Several potent neutralizing antibodies against DENV have been isolated from mice and humans, and the characterization of their properties by biochemical and biophysical methods have revealed important insights for development of therapeutic antibodies.
However, biophysical methods have recently begun to shed light on the structure and dynamics of transcription initiation in eukaryotes.
Size of lipid rafts, reported in the literature using a variety of biophysical methods, has ranged from 10-100 nm in diameter [ 4, 56, 57].
Many biophysical mechanisms have been proposed as feasible candidates in decoding a temporal code.
There is solubility near pH 3 and many biophysical measurements have been done at this pH.
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