Because the activation process of T cells is a key stage in T-cell mediated immune response, the investigation of biophysical changes of T cells could lead to further understanding of the mechanism of immune response.
In this study, atomic force microscopy (AFM) was carried out to detect the topographical and biophysical changes of nasopharyngeal carcinoma CNE-2Z cells and normal nasopharyngeal epithelial cells NP69-SV40T by treating the Disulfiram chelated with Cu2+ (DSF-Cu).
It has emerged as a powerful tool to measure the changes of mechanical property of cell membrane [5, 6, 8, 16 19], cell stiffness [8, 17], cell viscoelasticity [20, 21], and to measure the interaction between cells [1], by which one could get some valuable information about the biophysical changes of the activated lymphocytes.
We established a visual method by analyzing the changes of biophysical parameters of cells to reveal the characteristics and process of oncosis.
One particularly interesting property of YAP/TAZ is their capacity to sense and respond to changes in biophysical properties of cells including cell density, cell polarity, cell shape, tension forces, and substrate stiffness (Dupont et al., 2011; Halder et al., 2012; Aragona et al., 2013; Gaspar and Tapon, 2014; Rauskolb et al., 2014).
In the process of activation and proliferation of T lymphocytes, the variation of both cellular topography and membrane biophysical properties might correlate with the changes of biological function of T lymphocytes, such as the phosphorylation of signaling molecules, changes of cell polarity, and Ca2+ release [23 26].
The past decade has seen substantial growth in research into how changes in the biomechanical and biophysical properties of cells and subcellular structures influence, and are influenced by, the onset and progression of human diseases.
Further progress necessitates a synthesis - a conceptual understanding of how biophysical properties of cells may be interpreted by growing tissues into specific changes of growth and form.
In cryopreservation, the biophysical changes caused by the transition of water to ice during the cooling period are the main causes of cell damage [ 46].
