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The total protein contents of enzyme cocktails used in determining enzyme loadings on biomass were quantified using the Bradford Assay (Sigma Aldrich).
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In the FIA, belowground forest biomass is quantified using a root-shoot ratio [25].
The biomass was quantified using 180° backscattered light at 620 nm.
Fungal biomass was quantified using the lectin wheat germ agglutinin (WGA), which specifically binds to chitin, conjugated to fluorescein isothiocyanate (FITC) and leaf tissue was processed such that the levels of fluorescence detected in each sample were equivalent to the amount of chitin present [ 16].
Proteins were quantified using Qubit® 2.0 Fluorometer.
Immunoblots were quantified using ImageJ.
RNA samples were quantified using spectrophotometry.
PCR products were quantified using ImageJ.
Alternative splicing isoforms were quantified using ImageJ.
Images were quantified using Image J software.
Blots were quantified using ImageJ software.
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