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Green leaf number at collection or days to green-up, days to inflorescence production, leaf and inflorescence Cpi, and final B. tectorum biomass were analyzed using ANOVA with model effects of location, species (nested within location), community type, and their interactions.
Proteinogenic amino acids of the biomass were analyzed using gas chromatography mass spectrometry (GC-MS) technique according to Nanchen et al. with some modifications [ 45].
The effect of treatments on SSCE, soil moisture, soil temperature and above ground plant biomass were analyzed using analysis of variance (ANOVA) to test for significant differences using Sigma Stat [ 35].
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All biomass data were analyzed using Sigma Plot 11.
Variables characterising the entire plant such as total, shoot, vegetative and reproductive (flowers + pods) biomasses as well as root allocation were analyzed using one-way ANOVAs, with treatment as an independent factor.
DNAs enriched in bacterial sequences were produced from low-biomass oropharyngeal swabs using whole genome amplification and were analyzed using denaturing gradient gel electrophoresis analysis.
Raw material and WIS fraction were analyzed using the standard National Renewable Energy Laboratory (NREL) methods for determination of structural carbohydrates and lignin in biomass [ 39].
Images were analyzed using Gap Light Analyzer41.
Data were analyzed using one-way ANOVA.
Data were analyzed using Prism 5.0 (GraphPad Software).
Plates were analyzed using a Molecular Devices ImageXpress High-Content Analysis SysteMolecular Devices ImageXpress High-Content
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