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The amount of PHB in the bacterial biomass was determined using the method developed by Braunegg et al (1978).
The carbon content of the biomass was determined using a CHNS analyzer (Series II 2400 CHNS/O Perkin Elmer, Boston, USA).
Tree and shrub biomass were estimated using a regression model on the most dominant woody species while herbaceous biomass was determined using destructive sampling.
The total amount of Se in biomass was determined using inductively coupled plasma mass spectrometry (ICP-MS, Agilent 7700×) by a commercial company (Healthcare Institute Ltd., Ústí nad Labem, Czech Republic).
Heavy metal removal per unit biomass was determined using the following equation: M {text{uptake}}/{text{g}} = [M {text{Inf}}.) - M {text{Eff}}.)]/M {text{MLSS }} where M = uptake/g: heavy metal removed per unit mass of biomass, M (Inf).
A metal frame was used to mark the area in each case (Fig. 2); the weight of the plant biomass was determined using a potato sack and luggage scales.
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Minimum biofilm eliminating concentrations (MBEC) and biofilm biomass were determined using the crystal violet method.
The cellulose contents of the biomass were determined using National Renewable Energy Laboratory NRELL) methods [ 44] with minor modifications.
Protein and ammonium nitrogen contents in the biomass were determined using Kjeldahl assays and a Timberline TL-1800 analyzeranalyzerespectivelyely, at Dairy One Cooperative Inc. (Ithaca, NY).
The appearance of Pt(IV) challenging D02 biomass was observed by SEM examination; the reducing ratios of Pt(IV) to Pt II) and Pt II) to Pt(0) by the biomass were determined using XPS and the hydrolysates of the biomass for different time intervals were analyzed for glucose content via UV-vis spectrophotometry.
Algal biomass concentration was determined using the regression Eqs.
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