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Coronin 1A identified in the present study could be used as a biomarker for quantification of TILs in renal cancer.
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Camp et al. [10] have proposed a system called AQUA for quantification of biomarker expression based on FISH where specific fluorescent stains can be used for cell compartmentalization to detect nuclei, cytoplasm and membranes [11].
The data suggest that human vitreous body GFAP may be an interesting protein biomarker for indirect quantification of the glial response to retinal disorders.
Imaging-based biomarkers have recently been introduced for quantification of diffuse liver disease.
The subsequent bidirectional voltammetric analysis of stripping metal components from immunocomplexes for quantification of tumor biomarkers was performed in a microcell with minimum capacity of 50 μL.
The advantage of this approach is that it allows for quantification of several biomarkers simultaneously on the same tissue slide [ 53].
The satisfactory performances reached in terms of linearity, sensitivity (LOQs between 0.01 and 0.1 μg/mL), matrix effect (68 121%), accuracy, and precision (inter-day values between −24% and +16% and in the range 3.3 28.4%, respectively) make the proposed protocol suitable to be adopted for quantification of these important biomarkers in urine samples.
LC MS provides a promising alternative to ligand-binding assays for quantification of therapeutic proteins and biomarkers.
For instance, a chip-based, reversed-phase liquid chromatography-MALDI-MS analysis platform was utilized for quantification of glycomic-profile changes as cancer-biomarker discovery [ 32].
ImageJ (National Institutes of Health) was used for quantification of the immunolabeled nuclei.
A portable device for quantification of forearm muscle tone.
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