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Overall, individuals who were younger at diagnosis were more likely to be treated with biologics (data not shown).
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Its impact on the findings was evaluated in a sensitivity analysis and did not change the relative efficacy of abatacept to other biologic agents (data not reported).
Also, at D0, no difference was noted between survivors and nonsurvivors according to plasma IL-6, IL-8, and IL-10 levels and other biologic characteristics (data not shown).
Similar preparations and concentrations of steroids from the same supplier (Sigma) were tested by Dr. Wayne Meikle in the Division of Endocrinology at the University of Utah using an in-house estrogen and progesterone binding assay and a testosterone-dependent stimulation of prostate cancer cell apoptosis assay [ 12]; all the steroids retained biologic activity (data not shown).
In the TCZ group, 70% of the patients had previously used biologics; these patients had safety profiles similar to the biologics-naïve patients (data not shown).
Disease activity levels at baseline were distributed similarly among the APR groups in the subset of biologic-naïve patients with active RA (data not shown).
However, we initially focused on associations at the ≥ 0.01 significance level in the crude analyses (data not shown) and considered biologic plausibility.
This is supported by preliminary analyses of our database for 2012, which reveal prescription rates of biologic agents at the same level as 2011 (data not shown).
We found even greater degrees of aberrant gene splicing occurring in these other conditions (data not shown), thus reinforcing that important biologic functions relate to exon splicing.
Our own estimates from the biologics register show that only 4.6% of those receiving TNFi are treated exclusively privately (data not shown).
However, sensitivity analysis revealed that simply excluding patients with missing values and performing a univariate analysis for the effect of biologic class on response rates did not yield the same results as the multivariate approaches (data not shown).
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