RORA differential expression was not validated (p-value > 0.05) and, consequently, we did not perform biological validation on this sample.
Yamaguchi, H. et al. Peptidomic identification and biological validation of neuroendocrine regulatory peptide-1 and -2.
a, Biological validation of the TbFP by RNAi of procyclic trypanosomes, showing phylogenetic distribution and RNAi phenotype.
(b) Computation validation using COMSOL with Iset = 330 μA (c,d) Biological validation using MatLyLu single cell tracking.
To facilitate interpretation of these data, we provide detailed information on experimental design (Fig. 1), sequence quality control analyses (Fig. 2), and biological validation (Fig. 3).
As detailed biological validation of each individual target is a challenging, long and expensive process26,27, objective prioritization to a more manageable gene list is essential.
Biological validation demonstrated AMPKγ1 is also required for the survival of colon tumor cells, but not immortalized, non-transformed colon epithelial cells6.
Small-molecule chemical tools56 can substantially enhance biological validation and mechanistic evaluation, and in particular aid the understanding of the specific functional role of a protein in cancer.
Below, we briefly describe the rationale and considerations for sequencing data analysis with respect to general read quality, genome alignment, ChIP enrichment, replicate correlation, and biological validation.
Nicodemus, K. K. et al. Evidence of statistical epistasis between DISC1, CIT and NDEL1 impacting risk for schizophrenia: biological validation with functional neuroimaging.
This data set exemplifies large gene lists that have been generated from initial experimental exploration and that have varying degrees of biological validation.
