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Each sample was run in triplicate and 3 5 biological samples were run for each of the experimental conditions.
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Each biological sample was run in technical triplicates.
cDNA from each biological sample was run in triplicate, using specific primer pairs (available upon request).
Each biological sample was run in triplicate, RPL4 was used to standardize the relative expression of all genes investigated.
Fragment specificity was checked in melting curves and each biological sample was run in duplicate in the qRT-PCR.
Template cDNA corresponding to 4 ng of RNA was added to each PCR reaction and each biological sample was run in technical duplicates for each gene.
All samples were run in biological triplicates.
All samples were run with four biological replicates and three dilutions.
The Meis samples were run using two biological duplicates for the ChIP and matched-input DNA controls.
Samples were run from 5 independent biological replicates for each tissue type in TobEA.
All samples were run in triplicate, with at least three biological replicates.
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