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The biological samples were placed in a cuvette and sealed with Kapton tape as transmission windows.
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An optimum amount of biological sample was placed in the vial equipped with magnetic stirrer.
Prior to loading, the biological sample was placed in a solution of 50% H2O, 50% glycerol and 100 mM NaCl.
The microchip with the wet biological sample was placed with the silicon nitride facing up on the pole of the loading device and supported at two sides by a retractable aligner.
Silicone membranes serving as substitutes for biological tissue samples were placed inside a bioreactor [ 1] either in single-membrane or in double-membrane configuration.
The samples were placed in a FastPrep device (MP Biologicals) and disrupted for a total of 60 seconds (3 × 20 sec with 5 min cooling between them).
Samples were placed in polyethylene bags.
Samples were placed at 4 °C before imaging.
Serum samples were placed in designated microwells.
The samples were placed immediately on ice.
Also, when samples are placed in biological environments like blood plasma, β-TCP becomes unstable and gradually degenerates.
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