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Aliquots of all biological samples are processed (if applicable), frozen and stored for possible further disease-related testing.
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Biological samples were processed at the U.S. Naval Medical Research Center Detachment in Lima, Peru.
To avoid contamination or biological risk, the samples were processed in a class II biological safety cabinet that had been cleaned with 10percentt sodium hypochlorite and 70percentt alcohol, followed by exposure to ultraviolet light for 30 minutes, with the air circulation system turned on.
All samples were processed with four biological replicates.
Study samples were processed using Illumina Human Exome-12v1.1 Exome-12v1.1pass BeijingCompasshnology Co. Ltd; http://www.kangpusen.com/index.html).
In addition, samples were processed on ice to prevent hydrolysis and/or reaction of ECH and DCA in biological matrices.
All samples were processed individually.
Samples were processed within 1 h.
Samples were processed anonymous.
All samples were processed at CNIO.
All samples were processed in duplicate.
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