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No biological samples are assayed without the signed permission of the mother.
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The serum samples were tested for HBsAg using one ELISA kit (ETI-MAK-4, Diasorin, Italy), and all biological samples were assayed for all RTs evaluated.
For each genotype, three independent biological samples were assayed, each with two Illumina sequencing performance replicates, such that six replicates per genotype were sequenced.
A large number (over 200) of biological samples were assayed and the results were statistically processed by Frickenschmidt et al. [ 46].
Biological duplicate samples were assayed and differentially regulated genes were identified by comparing NPS stimulated NPSR1-A or -B transfected cells against NPS stimulated pCMV-vector transfected cells.
Three biologically independent samples were assayed for each condition.
Two independent P0 samples were assayed (P0 and P0', biological replicates) in order to evaluate the reproducibility of the experimental procedures.
A total of 100 μL of serum samples was assayed in parallel to known standard concentrations for each biological marker.
The samples were assayed on an ABI-prism 7500 Fast Applied Biosystemss).
Prenatal blood samples were assayed for cotinine.
Samples were assayed in quadruplicate.
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