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Two hundred nanograms of total RNA from each biological sample was used as starting material for miRNA expression analysis.
The three independent biological replicates for each age-group were analyzed by Scaffold analysis (version Scaffold 4.0.7, Proteome Software Inc., Portland, OR), and the label-free quantification of the protein expression in each biological sample was used to generate an unclustered heat map (Fig. 1e).
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At least two independent biological samples and three technical replicates of each biological sample were used for real-time PCR analysis.
Two technical replicates for biological sample were used and mean values were obtained for comparative analysis (Table S3 in Supplementary Material).
A single clinical isolation of Staphylococcus aureus (Boyd [1986]) from biological samples was used to produce focal infection.
RT information of 1946 newly identified lipids from four biological samples was used for validating that accurate precursor ion masses and MS/MS spectra were confirmed by RT matching.
cDNA prepared from triplicate biological samples was used for PCR analysis.
No direct measurement of PCBs in biological samples was used in analyzing disease risk associated with PCB exposure.
Analysis of steroids in biological samples is used routinely in the diagnosis of endocrine disorders.
Three independent biological samples were used with the gene-specific primers.
Three biological samples were used for each of the RNA and cDNA mixes.
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