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Acellular and protein-free simulated body fluid (SBF) used as a biological sample was prepared according to the chemical composition of a human body fluid, with ion concentrations almost equal to these of the inorganic constituents of human body plasma.
Acellular and protein-free simulated body fluid (SBF) used as a biological sample was prepared according to the chemical composition of a human body fluid, with ion concentrations nearly equal to those of the inorganic constituents of human body plasma.
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RNA from four biological samples was prepared for deep sequencing experiments: developing xylem and leaves of E. grandis BRASUZ1 and developing xylem of two unrelated E. globulus trees (named A2 and C3).
The biological samples were prepared by D.H., D.S.D.L., K.O. and M.Sve.
All biological samples were prepared by liquid liquid extraction and hesperetin was included as an internal standard (IS).
Biological samples were prepared in independent triplicates and amylase activity measurements of these samples were performed in technical triplicates for each plasmid.
Duplicate biological samples were prepared for mass spectrometry analysis as described previously (Whitney et al., 2014).
Three independent biological samples were prepared and an Arabidopsis Gene Expression Microarray (V4) of one color was used.
For each tissue type five biological samples were prepared by pooling 6 independent trees within each species.
Culture supernatants purchased from the American Tissue Culture Collection (ATCC) and other virus-containing biological samples were prepared for viral RNA and DNA extraction.
Biological samples were prepared by adding 8 mL nitric acid 70% ultra-pure (INSTRA-analyzed for trace metal analysis, Sodipro, Echirolles, France) and 2 mL of hydrogen peroxide.
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