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A cDNA panel of duplicates for each biological sample was constructed including: 16 necrotic lung, 14 demarcation zone lung, 16 unaffected lung, 8 adult, 8 F100, 8 F50, 8 trachea and 8 nose samples (43 biological samples in duplicates making 86 cDNA samples all together).
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To further shed light on the molecular mechanism by which physic nut seedlings respond to drought stress, gene expression profiles of a total of 24 samples (two biological replicates of each of 12 samples) were constructed.
Each construct was analyzed in three to seven biological replicates and each biological sample was measured in triplets.
A panel of three technical cDNA replicates for each of the three biological samples per condition was constructed.
A biological sample is a biological object such as blood.
An integrated biological network was constructed.
A model for amorphous sample D was constructed using Materials Studio 5.0 (Accelrys).
The measurement model of biological embedded engineering was constructed based on datadriven.
Furthermore, a biological signal cascade network was constructed based on these predicted target proteins.
We expect our method to be applicable to yeast in different sample conditions, since the gene network was constructed by integrating diverse biological experiments.
For each sample, an RNA sequencing library was constructed using the Illumina mRNA-Seq Prep Kit.
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