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The lack of concordance was also observed when biological replications were pooled and methylation of gene bodies or promoters clustered (data not shown).
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For each experimental variant 2-4 biological replicates were pooled.
Each biological replicate was pooled from 9 plants, and there were 3 biological replicates per treatment.
The leaf tissue of five replications was pooled to a total of two replications for each genotype - heat level combination to reduce biological variation for the following RNA sequencing.
Multiple tubes within a replication were pooled during protein extraction.
Leaf discs of eight leaves from two plants were pooled to make one replication and three biological replications were performed.
Two biological replications were conducted and the leaf samples from corresponding time points were pooled for ROS analysis and microarray experiments.
At least three technical and six biological replications were carried out for each experiment.
Three biological replications were employed.
Biological replications were harvested on different days.
For each sample, three biological replications were performed with each biological replication measured in three technique replications.
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