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Values from each biological replicate were transferred into the statistical analysis program GraphPad (GraphPad Software, Inc., La Jolla, CA, USA), verified for normality, and an analysis of variance (ANOVA) run to assay differences between experimental treatments.
For the first biological replicate, cells were transferred to a flask, methanol was added (50 mM) and time points were harvested at 0, 10, 30, 60, 120, 240 and 360 min post methanol addition, and each time point included a technical duplicate (dye-swap).
The germinated seeds were transferred into Petri dishes with filter paper and cultured in water (25 seedlings per dish, one biological replicate), and five independent biological replicates were employed, with two for sequencing and the other three for experimental verification.
At least two biological replicates were performed.
Four biological replicates were performed for each studied condition.
Three biological replicates were used for each treatment or control.
Four biological replicates were performed for each western blot assay.
Two biological replicates were prepared for each ChIP target.
Three biological replicates were collected for each stage.
Three biological replicates were collected at the same time.
Biological replicates were then merged using samtools (Version 1.4).
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