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The mapped reads from two biological replicates were subsequently analyzed by Cufflinks (version 2.1.1) to estimate the transcript abundance [ 75], and the expression level of each transcript was indicated as a FPKM value.
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One μg total RNA of every biological replicate was subsequently converted to cDNA using the iScript cDNA synthesis kit (Bio-Rad, http://www.bio-rad.com).
Two sets of root samples (biological replicates) were used to obtain two independent total RNA sample sets, and subsequently converted into two separate cDNA sample sets.
Subsequently, the mean and the standard error of the delta-CT of the three biological replicates were calculated.
Two biological replicates were used.
Three biological replicates were analyzed.
Three biological replicates were performed for each group.
Five biological replicates were analysed.
Three biological replicates were performed per group.
Three complete biological replicates were performed.
Three biological replicates were carried out.
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