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Twenty biological replicates were set for each paired group.
Sixteen biological replicates were set for the assay.
Three biological replicates were set for better coverage of the target proteome with reliable statistical consistency.
Since two biological replicates were set up, a total of 8 chips were used to compare transcript abundance per germination stage defined (see Figure 1).
Total RNA was extracted from quadriceps muscles of 10-week-old wt, Dysf −/−, F66, F66; Dysf −/−, and ACVR2B/Fc-injected wt and Dysf −/− mice (six different groups), and three biological replicates were set up for each group.
Previously, eight transcriptomes from roots of T. hispida treated with NaHCO3 for 0, 124 24 and 48 h (two biological replicates were set at each time point) were built [ 19].
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For each tests and control samples, two hybridizations were performed by using a reverse fluorescence strategy, and 1.8-fold averaged over the two biological replicates was set for significant change between differentiation and undifferentiating conditions of MSCs.
A biological replicate was set up with another 50 pots planted four months later to collect tissue in a similar way from each developmental stage.
Four sets of biological replicates were used for DNA microarray hybridization with Affymetrix Mouse Genome 430A 2.0 arrays (Affymetrix, Santa Clara, CA, USA).
Two sets of biological replicates were used for DNA microarray hybridization with the Affymetrix Rat Genome 430A 2.0 Arrays.
Triplicate sets of biological replicates were used for each experiment and each assay was repeated three times; statistical analysis utilized Minitab (version 14; Students T-test).
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