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Samples from seven biological replicates were separated on protein gels and each replicate gel lane was divided into fractions, each of which was analyzed by LC-MS/MS.
The embryo and endosperm collected from four germination periods with three biological replicates were separated and stored at −80°C prior to use.
Biological replicates were separated into two major clusters, one including the four GPC-RNAi samples and the other including the three wild type plants.
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Three biological replicates were measured, each containing 6 14 technical replicates.
Each biological replicate was separated on a single lane of an 8%T GELFrEE cartridge (6 lanes total).
Duplicate biological replicates were hybridized to separate arrays.
Biological replicates were generated by separating plants into two groups.
Two biological replicates were planted as separate blocks in an environmentally controlled growth chamber (Percival Scientific, Perry Iowa).
For each tissue type and sampling time-point, three biological replicates were combined for DGEP or separated for further expression analysis by qRT-PCR.
Biological replicates were prepared from two separate cultures of S. cerevisiae.
Two separate biological replicates were assayed for the clock shift assay and the assays using light to deprive flies of sleep; four separate replicates were performed for the mechanical sleep deprivation assays.
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