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Three independent biological replicates were selected for each time point for subsequent RNA sequencing.
These three fruits (biological replicates) were selected for volatile and microarray analyses.
Single phenotypically red or white colonies from at least three independently derived biological replicates were selected and grown overnight to a density of between 2.0 × 10 and 7.0 × 10 cells/ml.
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To equalise the sample numbers on both sides of the proteomic differential expression analysis a single fast growing clone (3 biological replicates) was selected at random and removed leaving 12 fast versus 12 slow samples (Additional file 1).
Three replicates were selected for each treatment.
For the YEL condition, 8 of 12 biological replicates were randomly selected and averaged two by two, establishing 4 independent measurements (for details see Additional file 4: Table S2).
Four biological replicates were used for each selected line.
For each scaffold condition, two biological replicates were chosen for analysis, deliberately selected such that local collagen wall orientation was kept constant between scaffold conditions.
Three biological replicates were performed for each of the selected genes.
Microarray data with intensities reproducibly higher than background from 10 array results of three biological replicate experiments were selected for analysis.
Two biological replicates were used.
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