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After qPCR confirmed expression of FaNES1, over-expression of FPS1L and HMGR1S and emission of nerolidol in COX + and COX++ transgenic lines (biological replicates) [ 17], the total RNA of 3 wild type plants, 3 COX + and 3 COX++ lines (biological replicates) were provided to ServiceXS (http://www.servicexs.com), who performed labelling, hybridization, quality control and data acquisition.
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The full dataset for two biological replicates is provided in File S1.
Four biological replicates were made, providing a total of twelve microarrays.
Seven independent biological replicates were used for each sample, which provided very strong statistical power - a false reading should only be obtained at the rate of 0.06%.
Three biological replicates were used to generate the graph provided in Figure 5.
Two biological replicates were used.
Three biological replicates were analyzed.
Three biological replicates were performed for each group.
Five biological replicates were analysed.
Three biological replicates were performed per group.
Three complete biological replicates were performed.
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