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In all experiments, samples from two or three biological replicates were processed in parallel to determine biological variability.
From each strain, two biological replicates were processed.
Two biological replicates were processed separately, and only sequence reads presented in both biological replicates were considered for further processing.
Each sample was run twice as a technical replicate, and five biological replicates were processed per experiment.
Four biological replicates were processed per species for all analyses except targeted HPLC-MS/MS, in which case only one replicate was performed.
Four separate biological replicates were processed for control and each of the blastema samples across the time course, and two were processed for the "opposite side" samples.
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Total RNA from four independent biological replicates was processed and hybridized to Agilent Human miRNA microarrays using standard protocols.
Three biological replicates were collected at each stage and were processed to generate indexed RNA-seq libraries, which were sequenced using an Illumina HiSeq 1000.
Briefly, total RNA samples from three biological replicates were pooled in equimolar concentrations and processed using the SOLiD total RNA-Seq Kit (Life Technologies), according to the manufacturer's instructions.
At least two biological replicates were performed.
Four biological replicates were performed for each studied condition.
More suggestions(17)
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biological replicates were normalized
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biological replicates were measured
biological replicates were used
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