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Three independent biological replicates were prepared for sequencing using the TruSeq Sample Preparation kit (Illumina) and sequenced on the Genome Analyser II system (GAIIx, Illumina).
Two biological replicates were prepared for each ChIP target.
In total, 12 samples (2 colours [Black/Red] × 2 developmental stages [24 h AP/72 h AP] × 3 biological replicates) were prepared for RNA-seq analysis.
Six libraries with two independent biological replicates were prepared using the Next Ultra Directional RNA Library Prep Kit NEBB) according to the manufacturer's instructions.
Three biological replicates were prepared for WT, cry1 and hfr1 individually for hybridization, scanning of the chips and further statistical analysis.
Three biological replicates were prepared.
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RNA (from three biological replicates) was prepared for arrays using the 3′ IVT Express Kit (Affymetrix, USA) as per manufacturer protocol 100 ng RNA and 15 amplification cycles.
A pool of all 15 samples (5 time points of 3 biological replicates) was prepared, labeled with Cy2, and used as a global internal standard.
Total RNA from three biological replicates was prepared from each yeast strain, hybridized to the array, and detected as described (Dutrow et al., 2008).
Two reverse transcription reactions (two technical replications) were prepared from each biological replicate.
Two biological replicates were independently prepared.
More suggestions(17)
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biological triplicates were prepared
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biological replicates were tested
biological libraries were prepared
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