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Probes with signal detection calls of P or A in both biological replicates were assigned consensus detection calls of PP and AA, respectively.
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Three replicated pastures or group pens with 3 steers per replicate were assigned into each treatment.
Three replicated pastures with 3 steers per replicate were assigned to each treatment.
Biological replicates were then assigned a single median value for each gene and time point.
EachRef-8 Beadchip can profile eight samples on a single slide in parallel, and therefore samples from three biological replicates were randomly assigned onto different slides.
Biological replicates were normalized using DESeq (Bioconductor) and RefSeq gene identifiers were assigned using biomaRt.
Two biological replicates were used.
Three biological replicates were analyzed.
Three biological replicates were performed for each group.
Five biological replicates were analysed.
Three biological replicates were performed per group.
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