Sentence examples for biological replicates was pooled from inspiring English sources

High quality RNA isolated from three independent tissue samples (biological replicates) was pooled for library preparation.

For each experiment, tissue from three biological replicates was pooled in.

The RNA of the biological replicates was pooled, lyophilized, and stored using the RNAstable® Tube Kit (Biomatrica, San Diego, CA, USA).

For sequencing, in case of each sample (0, 4, 8, and 12 DAP of Sitaphal or NMK-1), the equivalent quantity of total RNA of three biological replicates was pooled.

RNA quantity and quality was checked using bioanalyzer, and RNA from the 3 biological replicates was pooled in equal proportion to obtain one representative total RNA sample per phenotype (each made from 180 individuals).

For each sample, an equal amount of amplified mRNA from the three biological replicates was pooled for ds-cDNA synthesis following the protocol developed by the Schnable lab [ 52].

Briefly, RNA samples from the four biological replicates were pooled together, and subjected to DNase I treatment (Ambion, Cat. No. AM1907) until no DNA could be detected by qPCR.

For each experimental variant 2-4 biological replicates were pooled.

Three biological replicates were pooled in preparation of each of the six final samples.

Equal amounts of RNA from three biological replicates were pooled prior to cDNA preparation.

Biological replicates were pooled to make representative samples for deep sequencing analysis.