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Biotin-labelled cRNA from three biological replicates was generated from 250 ng of total RNA, hybridized onto MouseWG-6 version 2 Expression Bead Chips (Illumina, San Diego, CA) and data processed with the lumi Bioconductor package [56] including variance-stabilizing transformation and quantile normalization [57].
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In total, three biological replicates were generated for each strain.
Data from a minimum of three biological replicates were generated for each clone.
Detailed pair-wise scatter plots of biological replicates were generated for flag leaves (Figure 1A) and for seedling leaves (Figure S1A).
For both treatments, three independent biological replicates were generated.
Three biological replicates were generated at three different days.
Three independent biological replicates were generated for each genotype.
Biological replicates were generated by separating plants into two groups.
Biological replicates were generated as for RNA extraction.
Two biological replicates were generated and sequenced for mixed samples of five life cycle stages.
Biological replicates were generated by combining analysis of the three CF patients' samples.
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