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To quantify the transcripts, each cDNA of three biological replicates was diluted in duplicate and used in duplicate PCR.
Protein (300 μg) from three equally pooled biological replicates was diluted with 200 μl UA buffer (8 M Urea and 150 mM Tris HCl, pH8.0) and subjected to 30-kDa ultrafiltration.
Similar(58)
Each replicate was diluted 1 to 50 in T.E.
At the indicated doses, cultures (3 biological replicates) were appropriately diluted and plated on solid medium (8 g/L Bactotryptone, 4 g/L yeast extract, 3 g/L NaCl, pH 7.3, 2.8% Bactoagar), and CFU counts were determined after 2 days' incubation at 65°C.
Two biological replicates were used.
Three biological replicates were analyzed.
Results of 3 biological replicates are shown.
Three biological replicates were performed, similar results were obtained.
Three biological replicates were performed and similar results were obtained.
Three biological replicates were performed for each group.
Five biological replicates were analysed.
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