Sentence examples for biological replicates was determined from inspiring English sources
The mean of the two biological replicates was determined.
To identify WT1 responsive genes, the mean of two biological replicates was determined and the corresponding genes were selected (P < 0.05).
Average enrichment for all biological replicates was determined for each primer pair and standard deviation to the mean was indicated as error bars.
The quality of the biological replicates was determined by calculating R values among replicates of the same sample and the values ranged between 0.988 and 0.997 with an average of 0.995.
Two technical replicates were conducted for each biological replicate, and the average ± SE of three biological replicates was determined for each time point, which were arranged randomly in the controlled environment chamber.
The reproducibility between biological replicates was determined on total RNA isolated from three separate HeLa cell cultures, which were labeled with both Alexa546 and Alexa647 and hybridized on the array.
For each time point, two biological replicates of ethanol-treated samples and their control were analyzed and the corresponding gene expression ratios based on RPKM were calculated, the genes with 1.5 fold changes in both biological replicates were determined as differentially regulated genes.
A single gene-level value for each biological replicate was determined by calculating a weighted mean of all probes in each probe-set using the Tukey biweight formula, which minimizes the influence of outlier values.
All assays were performed in three technical replicates, and for each gene the average threshold cycle (Ct) value over all biological and technical replicates was determined.
Number of replicates were determined after an initial experiment with three biological replicates to gauge the data variation.
