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For our MapMan analysis, the average log2 value of both biological replicates was calculated for individual annotations in response to Fe deficiency and excess in roots and shoots.
The mean, standard deviation and relative quantity of replicates in relation to the reference population (RP, μ = 0.2 h−1, mean of two biological replicates) was calculated.
For data analysis, the arithmetic mean of two biological replicates was calculated.
Each reaction was performed in triplicate and the mean of three independent biological replicates was calculated.
To identify genes differentially expressed in the respective conditions, the average of at least two biological replicates was calculated.
Scattered plot matrices of biological replicates were generated and the Pearson product moment correlation coefficient between biological replicates was calculated using Bioconductor [ 14].
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Standard deviations of the mean value from three biological replicates were calculated as described previously.
The average surviving fraction from at least three biological replicates were calculated ±SEM.
Mean expression and standard error of three biological replicates were calculated for each treatment.
The average FPKM and average microarray intensity values of the biological replicates were calculated for all conditions.
To assess the reproducibility of microarray data, the correlation coefficients of biological replicates were calculated using GeneSpring GX 11 software.
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biological triplicates was calculated
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biological replicates was diluted
biological replicates was sequenced
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biological processes was calculated
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