Sentence examples for biological replicates was also from inspiring English sources
The reproducibility between two biological replicates was also high; the number of unique insertion sites/gene in MP1 and MP2 gave an R value of 0.984.
The parallel plot of these six RNA-Seq datasets after Loess normalization had a similar distribution pattern (Additional file 8C), and the correlation between the biological replicates was also very tight, with correlation coefficient values of 0.90, 0.91 and 0.87 for Phase I, II, III samples, respectively (Additional file 8D-F).
The RNA samples for microarray experiment (three biological replicates) were also used for real-time PCR assays in order to ensure the reliability and repeatability of the results.
Pearson correlation coefficients between biological replicates were also calculated and were in the range of 0.9812 to 0.9976 (Additional File 3), further corroborating significant correlations between biological replicates in each developmental stage.
The protein group ratios from three biological replicates were also analyzed in Perseus (version 1.3.0.4), using the Significance B method which considered both the protein ratio and intensities of the peptide ions.
In addition, the microarray data of biological replicates were also grouped together closely based on hierarchical clustering and principal components analyses (Additional file 2: Figure S1C, D), and the data can be grouped or separated as individual time points or by treatment.
The dispersion between each biological replicate was also tested by Cuffdiff, as well as the dispersion between each technical replicate.
However, in the case of the hybrids, mRNA from three separately extracted and amplified pools of 25 males were each used for hybridization to a single microarray, such that the replicates were also biological as well as technical, in order to determine whether there was a significant effect of between-extraction/amplification variability on our estimates of expression differences.
Dye-swap experiments (technical replicates) were also performed on each biological replicate.
