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Values from each biological replicate were transferred into the statistical analysis program GraphPad (GraphPad Software, Inc., La Jolla, CA, USA), verified for normality, and an analysis of variance (ANOVA) run to assay differences between experimental treatments.
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Manually validated hits from each of the three biological replicates were transferred to a relational database.
For the first biological replicate, cells were transferred to a flask, methanol was added (50 mM) and time points were harvested at 0, 10, 30, 60, 120, 240 and 360 min post methanol addition, and each time point included a technical duplicate (dye-swap).
Two biological replicate were done.
Two biological replicates for each sample and three technical replicates for each biological replicate were used for RT-PCR analysis.
Two biological replicates of each sample and three technical replicates per biological replicate were analyzed.
Four biological replicates and four technical replicates for each biological replicate were used.
Two biological replicates of each sample and three technical replicates of each biological replicate were used.
Each biological replicate was read in 2 technical replicates (TR).
Each biological replicate was scored at least in technical triplicate.
The germinated seeds were transferred into Petri dishes with filter paper and cultured in water (25 seedlings per dish, one biological replicate), and five independent biological replicates were employed, with two for sequencing and the other three for experimental verification.
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