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Four competitive hybridizations using the aphid line W109 (first biological replicate) were made as following: male → asexual female (long day) → sexual female → asexual female (short day) → male.
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Each biological replicate was made into its own DNA library.
Each biological replicate was made up of at least two technical replicates (within treatment samples).
Four biological replicates were made, providing a total of twelve microarrays.
And three biological replicates were made.
Two biological replicates were made for each time point.
Three biological replicates were made when culturing each of the six recombinant strains.
Two biological replicates were made for each condition: WT ESC and REST −/− ESC.
Three biological replicates were made per condition and formed one replicate group with average normalized spot intensities.
For each cell type, we isolated at least 1500 cell sections per biological replicate, and two independent biological replicates were made.
For the first experiment, for qRT-PCR as for microarray, three biological replicates were made for each tissue analyzed (i.e. leaves, flowers and mature beans).
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