Two biological replicate were done.
Two biological replicates for each sample and three technical replicates for each biological replicate were used for RT-PCR analysis.
Two or three biological replicates, with three technical replicates from each biological replicate were performed for each assayed gene.
Two biological replicates of each sample and three technical replicates per biological replicate were analyzed.
Four biological replicates and four technical replicates for each biological replicate were used.
Two biological replicates of each sample and three technical replicates of each biological replicate were used.
* Data from biological replicates were merged leaving 127 assays for analyses.
Each biological replicate was represented by bulks of leaves, stalk and secondary roots from five plants.
Each biological replicate was obtained by pooling three leaves (third leaf) harvested from three different plants (mock/infected) for each treatment.
Detailed pair-wise scatter plots of biological replicates were generated for flag leaves (Figure 1A) and for seedling leaves (Figure S1A).
