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20 to 25 female heads per biological replicate were homogenized in 100 µL of 70%% formic acid using a disposable pellet mixer and a plastic Eppendorf pestle.
20 female heads per biological replicate were homogenized in 100 µL 2× LDS containing reducing agent (Invitrogen) using a disposable pellet mixer and a plastic Eppendorf pestle.
Briefly, 20 female heads per biological replicate were homogenized in 100 µL ice-cold 1× PBS buffer supplemented with Complete mini without EDTA protease inhibitor (Roche) using a disposable pellet mixer and a plastic Eppendorf pestle.
Seeds from each biological replicate were homogenized in liquid nitrogen and approximately 100 mg of fresh tissue were extracted for 1 2 h with 1 mL of 50% ethanol.
Briefly, 20 female heads per biological replicate were homogenized in 100 µL ice-cold RIPA buffer (Pierce) supplemented with SDS at a final concentration of 1 % and Complete mini without EDTA protease inhibitor (Roche) using a disposable pellet mixer and a plastic Eppendorf pestle.
Similar(55)
All ground tissue from each biological replicate was homogenized and thoroughly mixed prior to RNA extraction procedures.
Two biological replicate were done.
Two biological replicates for each sample and three technical replicates for each biological replicate were used for RT-PCR analysis.
Two biological replicates of each sample and three technical replicates per biological replicate were analyzed.
Four biological replicates and four technical replicates for each biological replicate were used.
Two biological replicates of each sample and three technical replicates of each biological replicate were used.
More suggestions(15)
biological replicate were done
biological replicate were harvested
biological replicate were sliced
biological replicate were made
biological replicate were assessed
biological replicate were transferred
biological replicate were subjected
biological replicate was homogenized
biological replicate were mixed
biological replicate were labeled
biological replicate were given
biological replicate were amplified
biological replicate were performed
biological replicate were analyzed
biological replicate were averaged
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