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Data from each biological replicate were given as the mean of three technical replicates.
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The results of the real-time PCR analysis of two (tissues) and three (cells) biological replicates are given as mean ± S.E.M.
Mean log2 expression values of the four biological replicates are given in Table 1 and 2. Two-way analysis of variance (ANOVA) was used to test the effect of treatment (F1: bacterial response to iron deficiency versus control) and serotype (F2 response between serotypes 1, 2, 3, 5, 6 and 7) and the combined effect of treatment and serotype (F1 F2).
Two biological replicate were done.
Values are means ± SD of four biological replicates and are given as fold change compared to Ws-2 at 0 hr (left) or 4 hr (right).
Bars represent means ± SD of 5 biological replicates and are given as fold change to Ws-2 in L. (F ) Pictures of 5 week-old wt and mutant plants grown in a 12 hr light/12 hr dark cycle.
Each biological replicate was read in 2 technical replicates (TR).
Each biological replicate was scored at least in technical triplicate.
Given that independent biological replicates were used for each method, these differences could reflect the pathway-specific accumulation of additional gene expression changes.
For these nine different treatments, three biological replicates were carried out (independent RNAi, independent injury) giving a total of 27 samples.
The three biological replicates were combined by Tukey biweight function to give a single gene-level value for each of the five conditions and hierarchical clustering [ 60] was carried out on all 8110 probe-sets using the following parameters: Gene Tree selection only; Gene Leaf Order optimization; Euclidean Distance metric and complete linkage clustering.
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