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Three technical replicates (within each biological replicate) were employed for each tested sample and template-free negative controls.
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Differential expression measurements cannot be estimated with any confidence if a single biological replicate is employed.
Three biological replicates were employed during this study.
For each extraction three biological replicates were employed following the sample treatment [ 53] with some modifications.
Hence, three biological replicates were employed at each time point, two from the Trangie Merino flock and one from the CSIRO Merino × Romney flock.
Two biological replicates were employed for each stage and tissue for the 2005 samples, whereas one 2004 sample was used for each stage of mesocarp or exocarp.
The germinated seeds were transferred into Petri dishes with filter paper and cultured in water (25 seedlings per dish, one biological replicate), and five independent biological replicates were employed, with two for sequencing and the other three for experimental verification.
Three biological replications were employed.
Three biological and three technical replicates were employed per sample.
For each gene, three biological replicates and three technical replicates were employed.
Two biological replicate were done.
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