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Cells (40 ml for each biological replicate) were collected via centrifugation, and RNA was extracted using RNeasy Mini kits (Qiagen), followed by amplification with MessageAmp-II Bacteria RNA amplification kit (Ambion).
Samples for each biological replicate were collected from three maize plants and combined.
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Three biological replicates were collected for each stage.
Three biological replicates were collected at the same time.
mRNA and PolyRibo-3′ RNA-sequencing data from 4 biological replicates were collected to estimate variation and ensure reproducibility.
A total of five biological replicates were collected every 24 h during the time course (from day 0 to day 6) both in drought and watered conditions, and for each genotype (WT, quad, and BRL3ox).
Transcriptomes of mouse lens spanning seven developmental stages (three embryonic; E15, E15.5, E18 and four postnatal; P0, P3, P6, P9 stages with biological replicates) were collected from published sources for our study.
Three biological replicates were collected for each time point.
Two batches bacteria grown separately (biological replicates) were collected for this study.
All experiments were performed such that 4 biological replicates were collected for each strain in each condition.
Microarray analysis was performed using a custom Affymetrix chip and normalized hybridization values derived from three biological replicates were collected at each growth point.
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