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Exact(2)
However, the data and methods are not available for this study (due to citation of non-existent web pages), it is not clear that more than one biological replicate was tested, and the iron sources were acquired from a range of species.
Each sample of each biological replicate was tested three times.
Similar(58)
The mean of gene expression (log2 ratio) for three biological replicates was tested for significant difference against zero (no difference in gene expression between samples compared).
Statistical significance of the average fold changes across both biological replicates was tested using an adaptation of the method proposed by Willems et al. [ 24].
In each case, differential expression between three knockdown and three control samples (biological replicates) was tested using the Limma Ebayes algorithm.
With JMP® Genomics (Version 6.0, 64-bit Edition), hierarchical clustering on correlations between the biological replicates was tested with the Ward method.
Two biological replicates were tested for each condition.
For each growth condition, three independent biological replicates were tested.
Three independent biological replicates were tested in triplicates in the PCR experiments for the relative quantification of target genes.
For each condition, three biological replicates were tested and normalized to WT NR samples.
For each cDNA sample, technical duplicates in each of three biological replicates were tested.
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