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For the technical replicates, one of the biological replicates was split into three identical pools of RNA and hybridized independently.
One of the biological replicate was further split into three technical replicates.
Each biological replicate was assessed in three technical replicates.
One biological replicate was available per tissue.
Based on ZP birefringence and subsequent pregnancy results, CCs coming from each individual oocyte were considered as biological replicates and were split into two main experimental groups: - ZGP (Zona Good Pregnant) group: CCs of HZB oocytes that lead to successful pregnancy.
Two biological replicate were done.
Two biological replicates for each sample and three technical replicates for each biological replicate were used for RT-PCR analysis.
Two or three biological replicates, with three technical replicates from each biological replicate were performed for each assayed gene.
Two biological replicates of each sample and three technical replicates per biological replicate were analyzed.
Four biological replicates and four technical replicates for each biological replicate were used.
Two biological replicates of each sample and three technical replicates of each biological replicate were used.
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