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For each species, only one biological replicate was produced for both the anterior and the posterior midgut.
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In order to enable statistical analysis three biological replicates were produced by in vitro fertilization from different pairs of male and female frogs.
Four independent biological replicates were produced from each time points.
Three independent biological replicates were produced for the protein abundance quantitation in 1-DE, 2-De, and shotgun analyses.
For each cell-type, three biological replicates were produced, based on distinct rounds of plant cultivation and root embedding.
After three biological replicates were produced for each time point and pooled, RNA was sequenced producing a total of ~40 million paired reads for each time point.
In brief, 8 biological replicates were produced for each sample-type, and each sample was analysed using Taqman array cards, 384 well microfluidic arrays produced by Applied Biosystems.
Each cell line was cultured and passaged in the laboratory, and LMW RNA was isolated from a number of different passages so that four biological replicates were produced from each line.
Two technical replicates were produced for each biological replicate, for a total of eight hybridizations.
Two replicates were produced for each biological replicate, thus obtaining six gels per condition (n = 6).
Each biological replicate was sequenced twice on separate lanes on an Illumina Genome Analyzer I, producing a total of six replicates per genotype.
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