Sentence examples similar to biological replicate was normalized from inspiring English sources
Biological replicates were normalized using DESeq (Bioconductor) and RefSeq gene identifiers were assigned using biomaRt.
The expression levels across all 18 microarrays (for 6 conditions, each with three biological replicates) were normalized using the RMAExpress (version 1.0.5), implementing the Robust multiarray analysis (RMA) algorithm [ 33- 35].
Two sets of CEL files, created from 6 biological replicates and two sets of technical replicates were normalized using MAS 5.0 to expression signals, giving 6 values per probe/gene.
Each biological replicate was read in 2 technical replicates (TR).
Each biological replicate was scored at least in technical triplicate.
First, we compared the slope across biological replicates that were normalized with each of the two methods; because these are replicates, the slope across all genes is expected to be 1.0.
Two biological replicate were done.
Two biological replicates for each sample and three technical replicates for each biological replicate were used for RT-PCR analysis.
The microarray data was normalized by median scaling and the quality of the biological replicates was assessed by means of Pearson's coefficient.
The count data were normalized on the total number of counts taking the variance and the mean of the biological replicates was taken into account.
